Optimization of conditions for production of channel catfish ovary cells and channel catfish virus DNA.

Channel catfish reovirus (CRV) inhibits replication of channel catfish herpesvirus (CCV) by two distinct mechanisms: viral interference and induction of an anti-viral factor.

Catfish reovirus (CRV), a double stranded RNA virus, inhibited channel catfish herpes-virus (CCV) replication by 2 different mechanisms: (1) directly as a consequence of its own replication, and (2) indirectly due to the induction of an anti-viral factor. In the former, prior infection with CRV significantly reduced subsequent CCV protein synthesis and virus yield. CRV mediated-interference was...

Cloning and expression of a putative glycoprotein gene of channel catfish virus using baculovirus expression system.

The 1035 base-pair (bp) open reading frame 59 (ORF59) of channel catfish virus (CCV) codes for an abundant hydrophobic membrane glycoprotein considered to be the major envelope glycoprotein of the virus. The ORF59 gene of CCV was cloned into the baculovirus genome by site-specific transposition and expressed in Spodoptera frugiperda cells (Sf9 cells). Expressed protein could be recognized by po...

Epizootiology of Channel Catfish Virus Disease

Channel catfish virus disease (CCVD) is a highly pathogenic herpesvirus disease of cultured channel catfish, Ictalurus punctatus, fry and fingerlings. The disease is confined to the southern United States, but occurs sporadically in other areas where young channel catfish are intensively cultured. Blue catfish, I. furcatus, have been experimentally infected with CCV, but other ictalurids appear...

Short Communication:Effects of the addition of a marigold extract to diets fed to channel catfish (Ictalurus punctatus) on growth parameters

Detection of channel catfish virus DNA in latently infected catfish.

Channel catfish virus (CCV) disease is an acute haemorrhagic disease in juvenile channel catfish (Ictalurus punctatus). While fish that survive primary CCV infection are suspected of being carriers of CCV, little is known concerning CCV latency. In this report, fingerling catfish were infected with CCV by experimental immersion challenge. Infected fish displayed clinical signs of CCV disease, b...